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Updated: Aug 8, 2026

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
Published on: June 19, 2018
Better late than never for repair of miscoding lesions within a transcribed template
Erling Seeberg1, Arne Klungland
1Centre of Molecular Biology and Neuroscience and Department of Molecular Biology, Institute of Medical Microbiology, University of Oslo, The National Hospital, 0027 Oslo, , Norway.
Abstract:
Transcription does not always stall at base damage in DNA and can create mutated transcripts from miscoding lesions. In this issue of Molecular Cell, present genetic analysis of E. coli to indicate that the highly mutagenic purine modification, 8-oxoguanine, is subject to transcription-coupled repair despite transcriptional bypass and generation of mutant transcripts.
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