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Related Experiment Videos

A high-throughput screening system for genes extending life-span.

Cuiying Chen1, Sylviane Dewaele, Bart Braeckman

  • 1Fundamental and Applied Molecular Biology, Ghent University and Flanders Interuniversity Institute for Biotechnology, Ghent University, B-9000, Ghent, Belgium.

Experimental Gerontology
|October 29, 2003
PubMed
Summary

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Researchers developed a high-throughput screening method to identify genes that extend lifespan. This method identified ferritin light chain (FTL) as a gene that enhances stress resistance and prolongs life in yeast and C. elegans.

Area of Science:

  • Genetics
  • Molecular Biology
  • Aging Research

Background:

  • Understanding the genetic basis of aging is crucial for developing interventions.
  • High-throughput screening methods are essential for identifying novel genes involved in complex biological processes like aging.

Purpose of the Study:

  • To develop and validate a high-throughput functional genomic screening system for identifying lifespan-extending genes.
  • To investigate the role of identified genes in aging and stress resistance.

Main Methods:

  • Developed a yeast Saccharomyces cerevisiae screening system based on counting bud scars to identify long-lived mother cells.
  • Utilized fluorescently labeled Wheat Germ Agglutinin for chitin staining of bud scars.
  • Screened a human HepG2 cDNA expression library in yeast.

Related Experiment Videos

  • Identified and characterized the function of ferritin light chain (FTL) in yeast and Caenorhabditis elegans.

    • Main Results:

    • Successfully isolated 12 yeast transformants with potentially prolonged lifespans.
    • Identified ferritin light chain (FTL) as a key gene involved in lifespan extension.
    • Yeast cells overexpressing FTL exhibited increased resistance to iron and hydrogen peroxide, reduced cell death, and more cell divisions.
    • Overexpression of FTL in Caenorhabditis elegans led to an 8% increase in lifespan.

    Conclusions:

    • The developed high-throughput screening method is effective for identifying genes involved in aging processes.
    • Ferritin light chain (FTL) plays a conserved role in stress resistance and lifespan extension across species.
    • This research provides a valuable tool for aging research and the discovery of longevity-associated genes.