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Related Experiment Videos

One-step quantitative cortisol dipstick with proportional reading.

Wingman Leung1, Puiyee Chan, Freddy Bosgoed

  • 1Department of Chemistry, Hong Kong University of Science and Technology, Clear Water Bay Road, Clear Water Bay, Hong Kong, Kowloon, China.

Journal of Immunological Methods
|October 29, 2003
PubMed
Summary

A novel immuno-threshold assay rapidly quantifies cortisol in plasma within 5 minutes. This sensitive lateral flow assay offers high precision and low cross-reactivity, adaptable for whole blood and other small molecule detection.

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Area of Science:

  • * Immunoassay development
  • * Analytical chemistry
  • * Clinical diagnostics

Background:

  • * Traditional lateral flow assays often rely on competitive inhibition for hapten detection.
  • * Quantifying analytes like cortisol rapidly and accurately remains a challenge in point-of-care testing.

Purpose of the Study:

  • * To develop a rapid, quantitative immuno-detection method for cortisol using a lateral flow assay.
  • * To establish an immuno-threshold-based assay for direct signal proportionality to cortisol concentration.
  • * To adapt the assay for both plasma and whole blood samples.

Main Methods:

  • * Development of an immuno-threshold-based lateral flow assay for cortisol quantification.
  • * Characterization of assay performance including detection limit, precision, and cross-reactivity.

Related Experiment Videos

  • * Adaptation of the assay for a one-step whole blood cortisol test.

    • Main Results:

    • * The plasma assay provides quantitative cortisol results in 5 minutes with a detection limit of 3.5 ng/ml.
    • * High precision was achieved with intra-assay CV of 6% and inter-assay CV of 10%.
    • * Low cross-reactivity with related steroids was observed; a 15-minute whole blood assay was successfully developed.

    Conclusions:

    • * The immuno-threshold lateral flow assay offers a rapid, sensitive, and precise method for cortisol quantification.
    • * The assay format is stable, suitable for mass production, and adaptable for detecting other low-molecular-weight haptens and drugs.