Liquid chromatography-electrospray mass spectrometry (LC-MS) method for determination of esmolol concentration in
Athena F Zuppa1, Heng Shi, Peter C Adamson
1Division of Clinical Pharmacology and Therapeutics, Department of Pediatrics, The Children's Hospital of Philadelphia, Abramson Research Center, Suite 907, 3615 Civic Center Blvd., Philadelphia, PA 19104-4318, USA.
Abstract:
Esmolol is a very short-acting beta-blocker commonly used in critically ill patients. To study the pharmacokinetics of esmolol in pediatric patients, a liquid chromatography-electrospray mass spectrometry (LC-MS) method to quantitate esmolol concentration in plasma was developed. Following methylene chloride extraction from 200 microl aliquots of plasma containing internal standard and reconstitution in 0.05% formic acid, 10 microl are injected onto column, eluted using a methanol/formic acid gradient over 15 min, and monitored with selected ion recording at 296.2 and 282.2. The assay is linear between 2 and 1000 ng/ml. The intra-day and inter-day coefficients of variation are less than 8 and 10%, respectively.
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