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Measuring Changes in Tactile Sensitivity in the Hind Paw of Mice Using an Electronic von Frey Apparatus
Published on: December 19, 2013
5-hydroxytryptamine1A receptor is involved in the bee venom induced inflammatory pain
Wen Wang1, Sheng-Xi Wu, Ya-Yun Wang
1Department of Anatomy and K.K. Leung Brain Research Centre, The Fourth Military Medical University, 17 West Chang'le Road, Xi'an 710032, People's Republic of China.
Abstract:
Injection of bee venom into one hindpaw of rat can elicit acute inflammation together with spontaneous pain, heat hyperalgesia and mechanical hyperalgesia/allodynia in the injected paw. 5-hydroxytryptamine (5-HT)1A receptor is the predominant receptor subtype in the spinal dorsal horn mediating the function of 5-HT in nociception. The goal of the present study is to assess the role of 5-HT1A receptor in the pain associated with the bee venom induced inflammation. Here we showed that 1 or 4 h after a subcutaneous bee venom challenge, expression of 5-HT1A receptor mRNA in the ipsilateral lumbar spinal cord increased significantly by 80.94 or 37.86%, respectively. Antisense oligodeoxynucleotide knockdown of spinal 5-HT1A receptor attenuated spontaneous pain and reversed heat hyperalgesia in rats injected with bee venom. Thus, the present data suggest a facilitating role for 5-HT1A receptor in bee venom induced inflammatory pain.
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