Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A three-dimensional viscoelastic model for cell deformation with experimental verification.

Hélène Karcher1, Jan Lammerding, Hayden Huang

  • 1Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

Biophysical Journal
|October 29, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polarization increases nuclear stiffness in macrophages despite reduction in lamin A/C levels.

npj biological physics and mechanics·2026
Same author

Antagonistic contributions of A-type and B-type lamins to LBR localization and dynamics.

Nucleus (Austin, Tex.)·2026
Same author

Deep Invaginations of Nuclear Envelope Coordinate Spatial Organization of Chromatin in Epithelium.

bioRxiv : the preprint server for biology·2026
Same author

Antagonistic contributions of A-type and B-type lamins to LBR localization and dynamics.

bioRxiv : the preprint server for biology·2026
Same author

A-type lamins anchor emerin at the inner nuclear membrane via two independent binding sites.

The Journal of biological chemistry·2026
Same author

A subnanolitre tetherless optoelectronic microsystem for chronic neural recording in awake mice.

Nature electronics·2025

A new 3D finite element model analyzes cell mechanics during magnetocytometry. It reveals localized stress and strain, showing cell cytoskeleton properties are key to understanding cell manipulation.

Area of Science:

  • Biophysics
  • Cell Mechanics
  • Biomaterials

Background:

  • Cell micromanipulation techniques are crucial for biological studies.
  • Magnetocytometry offers a non-invasive method for cell manipulation.
  • Understanding cell mechanical properties is essential for interpreting magnetocytometry results.

Purpose of the Study:

  • To develop a 3D viscoelastic finite element model for cell micromanipulation using magnetocytometry.
  • To analyze strain and stress fields induced in cell monolayers during magnetocytometry.
  • To validate the model against experimental data.

Main Methods:

  • Developed a 3D viscoelastic finite element model.
  • Modeled cell membrane/cortex and cytoskeleton as Maxwell viscoelastic materials.

Related Experiment Videos

  • Validated numerical predictions against experimental data on NIH 3T3 fibroblasts.

    • Main Results:

    • The model accurately predicts strain/stress fields in cell monolayers during magnetocytometry.
    • Cell membrane/cortex effects were negligible on magnetocytometry timescales.
    • Stress and strain patterns were highly localized (<10 microm from the bead).
    • NIH 3T3 fibroblasts showed a viscoelastic timescale of ~1 s and shear modulus of ~1000 Pa.

    Conclusions:

    • The developed model is a robust tool for analyzing cell mechanics in magnetocytometry.
    • Magnetocytometry effects are localized, primarily impacting the cytoskeleton.
    • Cell mechanical properties, particularly the cytoskeleton's viscoelasticity, are critical for magnetocytometry applications.