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Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
Published on: June 26, 2018
Expression of bacterial beta-galactosidase in animal cells
G An1, K Hidaka, L Siminovitch
1Department of Medical Genetics, University of Toronto, Hospital for Sick Children, Toronto, Ontario, Canada.
Molecular and Cellular Biology
|December 1, 1982
Abstract:
A recombinant plasmid containing the gene for bacterial beta-galactosidase, situated close to the simian virus 40 early promoter, has been constructed. Transfection of CHO, L, and COS-1 cells with this plasmid led to the expression and appearance of the enzyme. Using this system, we have developed a series of promoter cloning vehicles capable of accepting promoter signals for animal genes.
