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Updated: Aug 30, 2026

Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves
Published on: March 9, 2014
Development of a system for the study of protein-protein interactions in planta: characterization of a TATA-box
Jingping Zhong1, Paul A Haynes, Shiping Zhang
1Department of Functional Genomics, Torrey Mesa Research Institute, Syngenta Research and Technology, San Diego, California 92121, USA.
Abstract:
We describe a simple, rapid method for protein complex purification in planta. Using a biotin peptide as an affinity tag with TATA-box binding protein (TBP), 86 unique proteins present in the purified complex were identified by tandem mass spectrometry. We identified proteins known to be associated with TBP, and many other proteins involved in pre-mRNA processing and chromatin remodeling. The identification of these novel protein-protein associations will upon further investigations provide new insights into the mechanisms of mRNA transcription and pre-mRNA processing.
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