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Related Experiment Videos

Does axonemal dynein push, pull, or oscillate?

Charles B Lindemann1, Alan J Hunt

  • 1Department of Biological Sciences, Oakland University, Rochester, Michigan 48309, USA. lindeman@oakland.edu

Cell Motility and the Cytoskeleton
|October 30, 2003
PubMed
Summary
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Dynein motors in cilia and flagella may use a tension-regulated, anchored head mechanism for force generation. This model explains flagellar bending and reconciles dynein oscillation with interdoublet sliding.

Area of Science:

  • Biophysics
  • Molecular Biology
  • Cell Biology

Background:

  • Dynein is a molecular motor essential for cilia and flagella motility.
  • It consists of a globular head, stem, and stalk (B-link) connecting microtubule doublets.
  • The B-link is hypothesized to mediate interdoublet force transfer for flagellar bending.

Purpose of the Study:

  • To investigate the mechanism of force generation by dynein motors.
  • To evaluate the role of B-link and stem elasticity in force transmission.
  • To propose a model for dynein cross-bridge cycling that explains flagellar motility.

Main Methods:

  • Analysis of published data on dynein motor stiffness (Burgess et al., 2003).
  • Calculation of force transfer capabilities based on B-link and stem elasticity.

Related Experiment Videos

  • Theoretical modeling of dynein cross-bridge mechanics.

    • Main Results:

    • The calculated stiffness of the B-link and stem suggests limitations in force transfer if the stem is the sole support for the dynein head.
    • A stem-supported model predicts insufficient force for flagellar motility.
    • Lateral displacement of the B-link is a more plausible force generation mechanism, requiring additional head stabilization.

    Conclusions:

    • Dynein force generation likely involves more than simple linear force transmission through the stem and B-link.
    • A model incorporating an anchored head and tension-regulated detachment of the B-link is proposed.
    • This mechanism may unify dynein oscillation and interdoublet sliding within a single cross-bridge cycle.