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Reusing soy residue for the solid-state fermentation of Ganoderma lucidum
Chienyan Hsieh1, Fan Chiang Yang
1Department of Bioindustry Technology, DaYeh University, 51505 Chang-Hwa, Taiwan, ROC. mch@mail.dyu.edu.tw
Bioresource Technology
|October 31, 2003
Summary
Soy residue can be used to culture Ganoderma lucidum (Lingzhi) mushrooms. Optimal growth and higher nutritional content in fruiting bodies were achieved using specific carbon/nitrogen ratios and containers.
Area of Science:
- Mycology
- Biotechnology
- Traditional Medicine
Background:
- Lingzhi (Ganoderma lucidum) is a traditional oriental medicine with diverse therapeutic applications.
- Soy residue, a byproduct of tofu manufacturing, presents a potential substrate for fungal cultivation.
- Solid-state fermentation (SSF) is an efficient method for culturing fungi on solid substrates.
Purpose of the Study:
- To investigate the efficacy of soy residue as a substrate for Ganoderma lucidum cultivation using SSF.
- To determine the optimal carbon/nitrogen (C/N) ratio and container type for Ganoderma lucidum growth and fruiting body development.
- To analyze and compare the nutritional composition of Ganoderma lucidum fruiting bodies cultured under different conditions.
Main Methods:
- Ganoderma lucidum was cultured in soy residue using solid-state fermentation.
- Fermentation was performed in three types of containers: test tubes, 500-ml flasks, and polypropylene plastic bags.
- Various carbon/nitrogen (C/N) ratios were tested to optimize mycelial growth and fruiting body formation.
- Components of the fruiting bodies, including ash, polysaccharides, and crude protein, were analyzed.
Main Results:
- The highest mycelial growth rate (6 mm/day) was observed in test tubes with a C/N ratio of 80.
- A higher growth rate (7.5 mm/day) was achieved in 500-ml flasks with C/N ratios of 70-80.
- Complete fruiting body development occurred in plastic bags at C/N ratios of 70 and 80.
- Fruiting bodies cultured with a C/N ratio of 80 exhibited higher contents of ash, polysaccharides, and crude protein.
Conclusions:
- Soy residue is a viable substrate for Ganoderma lucidum cultivation via solid-state fermentation.
- The choice of container and C/N ratio significantly impacts mycelial growth, fruiting body development, and nutritional yield.
- A C/N ratio of 80, particularly in conjunction with appropriate containers, favors enhanced nutritional quality of Ganoderma lucidum fruiting bodies.