Related Experiment Video
Updated: Aug 30, 2026

Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
Published on: August 25, 2019
The effect of pronuclear morphology on early development and chromosomal abnormalities in cleavage-stage embryos
Pilar Gámiz1, Carmen Rubio, M José de los Santos
1Instituto Valenciano de Infertilidad (IVI), Plaza Policía Local, Valencia, Spain.
Background:
Pronuclear (PN) zygote morphology has been proposed as a useful tool for selecting the best embryos for transfer.
Methods:
PN morphology was recorded in 888 zygotes and classified according to similar/different PN size [groups A (n = 816) and B (n = 72)] and to the number, distribution and synchrony of nucleolar precursor bodies (NPB): subgroup I, pronuclei with 3-4 polarized NPB; subgroup II, 5-7 synchronic polarized NPB or 7-10 NPB distributed randomly; and subgroup III, morphologies other than those of groups I or II. Embryo development and chromosomal abnormalities were evaluated for each PN pattern.
Results:
In patients aged =37 years, the number of zygotes reaching morula and blastocyst stage was significantly (P = 0.0003) higher in group A than in group B. In group A, the incidence of chromosomal abnormalities was significantly (P = 0.0247) lower than in group B, and significant differences were observed when pattern AI was compared with pattern AII (P = 0.0280), AIII (P = 0.0024), BIII (P = 0.0077) and total B (P = 0.0247). In patients aged >37 years, statistical differences among groups were not observed.
Conclusions:
In patients aged =37 years, zygotes with similar PN size and with polarized NPB present the best prognosis based on embryo development and the incidence of chromosomal abnormalities, whereas in patients aged >37 years, this correlation does not exist.
More Related Videos
12:32Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
Published on: September 7, 2021
08:43Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
Published on: June 6, 2016
Related Concept Videos
Cleavage and Blastulation
Nondisjunction
Nondisjunction
Teratogenicity
Meiosis vs. Mitosis
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
Nucleosome Remodeling
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...