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Updated: Aug 17, 2026

Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
Published on: January 7, 2019
Regulation of T-cell activation by phosphodiesterase 4B2 requires its dynamic redistribution during immunological
Jacqueline Arp1, Mark G Kirchhof, Miren L Baroja
1Robarts Research Institute, Department of Microbiology and Immunology, The University of Western Ontario, London, Ontario, Canada N6A 5K8.
Abstract:
Stimulation of T cells through their antigen receptors (TCRs) causes a transient increase in the intracellular concentration of cyclic AMP (cAMP). However, sustained high levels of cAMP inhibit T-cell responses, suggesting that TCR signaling is coordinated with the activation of cyclic nucleotide phosphodiesterases (PDEs). The molecular basis of such a pathway is unknown. Here we show that TCR-dependent signaling activates PDE4B2 and that this enhances interleukin-2 production. Such an effect requires the regulatory N terminus of PDE4B2 and correlates with partitioning within lipid rafts, early targeting of this PDE to the immunological synapse, and subsequent accumulation in the antipodal pole of the T cell as activation proceeds.
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