A novel selection system for potato transformation using a mutated AHAS gene
M Andersson1, A Trifonova, A-B Andersson
1Plant Science Sweden AB, Herman Ehles Väg 2-4, 268 31 Svalöv, Sweden. Mariette.Andersson@plantscience.se
Plant Cell Reports
|October 31, 2003
Summary
A mutated Acetohydroxyacid synthase (AHAS) gene enhances herbicide tolerance in potato transformation. This novel selection method achieved high transformation efficiency and produced potato lines with improved starch quality.
Area of Science:
- Plant biotechnology
- Molecular genetics
- Agricultural science
Background:
- Acetohydroxyacid synthase (AHAS) is a key enzyme targeted by herbicides.
- A specific mutation (S653N) in the AHAS gene confers tolerance to imidazolinone herbicides.
Purpose of the Study:
- To evaluate the efficacy of the mutated AHAS gene as a selection marker in potato transformation.
- To develop a highly efficient potato transformation system and produce high-amylopectin potato lines.
Main Methods:
- Introduction of the mutated AHAS gene and a GUS reporter gene into potato.
- Selection of transformed potato shoots using the herbicide Imazamox.
- Analysis of GUS expression and starch quality in regenerated potato lines.
Main Results:
- The mutated AHAS gene facilitated a transformation system with very high frequency and minimal escapes.
- Selection with 0.5 microM Imazamox resulted in GUS expression in 93-100% of regenerated shoots.
- Successfully produced potato lines exhibiting desirable high-amylopectin starch quality.
Conclusions:
- The mutated AHAS gene is a highly effective selection marker for potato transformation.
- This method enables efficient production of genetically modified potatoes with improved starch characteristics.
