A high mobility group protein from the Dipteran insects Ceratitis capitata and Bactrocera oleae
Related Experiment Video
Updated: Aug 30, 2026
Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata
Published on: October 1, 2016
Vassiliki Aleporou-Marinou1, Christina Deli, Yiannis Ninios
1Department of Biology, Division of Genetics and Biotechnology, University of Athens, Panepistimiopolis 15701, Athens, Greece. valepor@biol.uoa.gr
Abstract:
Nuclei from Bactrocera oleae and Ceratitis capitata larvae contain a major protein that shares most of the characteristics of vertebrate high mobility group (HMG) proteins. Proteins are extracted from nuclei with 0.35 M NaCl, are soluble in 5% perchloric acid, are relatively small (molecular weight in the range of 10-16 kDa), and have both a high basic and a high acidic amino acid content. The amino acid constitution of these proteins is similar to that of the HMGB protein family of vertebrates. The proteins cross-react with antibodies raised against the HMGD chromosomal protein of Drosophila melanogaster. The possible relatedness of these proteins to high mobility group proteins is discussed.
