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Rapid screening by MALDI-TOF mass spectrometry to probe binding specificity at enzyme active sites
Michael E Webb1, Elaine Stephens, Alison G Smith
1University Chemical Laboratory, University of Cambridge, Lensfield Road, Cambridge, UK CB2 1EW.
Chemical Communications (Cambridge, England)
|November 1, 2003
Abstract:
The binding affinity of aspartate decarboxylase has been probed using MALDI-TOF spectrometry; adducts formed covalently in the active site were detected by MALDI-TOF mass spectrometry after incubation of the enzyme with a range of potential ligands in the presence of NaCNBH3; this has highighted key structural features which will aid design of potential inhibitors.