Related Experiment Video

Updated: Aug 30, 2026

Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice
08:02

Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice

Published on: October 19, 2013

The role of endogenous NADPH oxidases in airway and pulmonary vascular smooth muscle function

John R Hoidal1, S S Brar, Anne B Sturrock

  • 1Department of Internal Medicine, University of Utah School of Medicine, Medical Service, Salt Lake City, UT, USA. john.hoidal@hsc.utah.edu

Antioxidants & Redox Signaling
|November 1, 2003
PubMed

Insights

Reactive oxygen species from NADPH oxidases are key in airway and pulmonary artery smooth muscle cells. Nox4 is predominant, but expression varies with cell function, impacting asthma and vascular diseases.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Physiology

Background:

  • Reactive oxygen species (ROS) generated by NADPH oxidases (NOX) are crucial signaling molecules in smooth muscle cells.
  • Nox4 is identified as a primary gp91 homologue in airway smooth muscle cells (ASMCs) and pulmonary artery smooth muscle cells (PASMCs).

Purpose of the Study:

  • To investigate the role and regulation of NADPH oxidase(s) in ASMCs and PASMCs.
  • To understand the implications of NOX activity in the pathobiology of asthma and pulmonary vascular diseases.

Main Methods:

  • Analysis of NADPH oxidase component expression in ASMCs and PASMCs.
  • Assessment of NOX-dependent signaling pathways related to cell proliferation and oxygen sensing.

Main Results:

  • NADPH oxidase activity is implicated in the mitogenesis of ASMCs and PASMCs.
  • NOX enzymes play a significant role in cellular oxygen sensing mechanisms.
  • Expression patterns of NADPH oxidase components are dependent on cell phenotype and functional state.

Conclusions:

  • NADPH oxidase(s), particularly Nox4, are vital for normal function in airway and pulmonary artery smooth muscle cells.
  • Dysregulation of NOX in these cells contributes to the pathogenesis of asthma and pulmonary vascular diseases.
  • Targeting NOX pathways may offer therapeutic strategies for respiratory and cardiovascular conditions.

More Related Videos

Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle08:59

Utilizing the Precision-Cut Lung Slice to Study the Contractile Regulation of Airway and Intrapulmonary Arterial Smooth Muscle

Published on: May 5, 2022

Isolation of Intrapulmonary Artery and Smooth Muscle Cells to Investigate Vascular Responses07:56

Isolation of Intrapulmonary Artery and Smooth Muscle Cells to Investigate Vascular Responses

Published on: June 8, 2022

Related Concept Videos

Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...
Chronic Obstructive Pulmonary Disease-II: Pathophysiology01:20

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
Autoregulation of Blood Flow01:17

Autoregulation of Blood Flow

Autoregulation mechanisms are characterized by their inherent capacity for self-regulation without necessitating specific nervous stimulation or endocrine control. These mechanisms facilitate the adjustment of blood flow and, therefore, perfusion specific to each tissue region. This self-regulation encompasses chemical signals and myogenic controls.
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation.
Physiological Control of Respiration01:23

Physiological Control of Respiration

Introduction
Breathing, a seemingly passive process, is regulated by the respiratory center in the brainstem. This center coordinates the involuntary control of respirations, which means it occurs without conscious effort, ensuring a smooth and uninterrupted pattern.
Regulation of Ventilation
The body maintains ventilation by monitoring levels of carbon dioxide (CO2), oxygen (O2), and hydrogen ion concentration (pH) in the arterial blood. Among these factors, the level of CO2 plays a crucial...
Pulmonary Hypertension: Classification and Pathogenesis01:30

Pulmonary Hypertension: Classification and Pathogenesis

Pulmonary hypertension (PH) is a severe health condition in which the mean pulmonary arterial pressure increases to 25 mmHg or more, even when the body is at rest. This high pressure in the blood vessels that transport blood from the heart to the lungs can cause various symptoms, including shortness of breath, can lead to right heart failure, and significantly affect the overall quality of life.
There are various classifications for PH, each relating to different underlying causes and also...
Structure of Blood Vessels01:15

Structure of Blood Vessels

Blood is circulated throughout the human body through a network of blood vessels called the circulatory system. This system includes arteries that transport blood from the heart to various body parts. These arterial pathways divide into smaller vessels until they reach the arterioles, which further split into capillaries. It is within these minuscule capillaries that the exchange of nutrients and waste products takes place. After this exchange, the blood is collected by venules, which fuse to...