Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Manual sequencing using pulsed field.

C Turmel1, E Brassard, J Noolandi

  • 1Xerox Research Centre of Canada, Mississauga, Ontario.

Electrophoresis
|September 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preparation, Manipulation, and Pulse Strategy for One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE).

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Preparation and Separation of Intact Chromosomes of Vertebrates by One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE).

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Elastic Bag Model of One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE).

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Electronic structure of dinuclear gold(i) complexes.

Metal-based drugs·2008
Same author

Streptavidin tetramerization and 2D crystallization: a mean-field approach.

Biophysical journal·2001
Same author

Meanfield approach to the thermodynamics of protein-solvent systems with application to p53.

Biophysical journal·2001
Same journal

Kinship Inferences for Second-Degree Relatives With a Combination of STRs and Microhaplotypes.

Electrophoresis·2026
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
See all related articles

Pulsed field gel electrophoresis enhances DNA sequencing resolution, extending read lengths to thousands of bases. This method overcomes band inversion and improves band sharpness for accurate DNA fragment analysis.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genomics

Background:

  • Traditional direct current (DC) field gel electrophoresis in DNA sequencing faces limitations in resolution and band inversion.
  • Achieving accurate visual reading beyond a few hundred bases is challenging with standard methods.

Purpose of the Study:

  • To investigate the application of pulsed field electrophoresis for improving DNA sequencing resolution.
  • To overcome the band inversion problem and enhance band sharpness in DNA fragment analysis.

Main Methods:

  • Utilizing pulsed electric fields instead of a constant DC field during gel electrophoresis.
  • Optimizing pulsing conditions to manage band inversion and improve resolution.
  • Employing continuous band spectrum stretching during migration for enhanced analysis.

Related Experiment Videos

Main Results:

  • Extended the resolution range from hundreds to several thousand bases.
  • Successfully overcame the band inversion problem inherent in DC field methods.
  • Achieved sharper bands compared to DC field electrophoresis, enabling visual reading up to 800-900 bases.
  • Demonstrated compatibility with automation techniques due to continuous stretching of the band spectrum.

Conclusions:

  • Pulsed field electrophoresis significantly improves DNA sequencing resolution and accuracy.
  • The method offers a viable alternative to DC field electrophoresis for longer DNA fragment analysis.
  • This technique is adaptable for automated DNA sequencing workflows.