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A PacI/SwaI map of the Pseudomonas aeruginosa PAO chromosome
1Abteilung Biophysikalische Chemie, Medizinische Hochschule, Hannover, Germany.
Electrophoresis
|September 1, 1992
Abstract:
The PacI/SwaI macrorestriction map of the 5.9 Mb Pseudomonas aeruginosa PAO (DSM 1707) genome was constructed by fragment size comparisons of complete single and double digests and hybridization with probes of known genomic localization. P. aeruginosa PAO contains five recognition sites for PacI and six sites for SwaI. An integrated DpnI/PacI/SpeI/SwaI map was obtained by fragment pattern and Southern blot hybridization analyses of all combinations of double digestions and by two-dimensional pulsed-field gel electrophoresis mapping protocols.