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Related Experiment Videos

Plasma protein map: an update by microsequencing.

G J Hughes1, S Frutiger, N Paquet

  • 1Medical Biochemistry Department, Geneva University, Switzerland.

Electrophoresis
|September 1, 1992
PubMed
Summary
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This study presents an updated human plasma protein map using two-dimensional electrophoresis and microsequencing. Over 40 polypeptide chains were identified, enhancing the human plasma two-dimensional gel database.

Area of Science:

  • Proteomics
  • Biochemistry
  • Molecular Biology

Background:

  • Accurate protein identification is crucial for understanding biological processes.
  • Existing human plasma protein databases require continuous updates with new discoveries.

Purpose of the Study:

  • To present a reference map of human plasma proteins.
  • To identify and catalog novel polypeptide chains in human plasma.
  • To update the existing two-dimensional gel database for human plasma proteins.

Main Methods:

  • Two-dimensional electrophoresis utilizing immobilized pH gradients.
  • Microsequencing for polypeptide chain identification.

Main Results:

  • A comprehensive reference plasma protein map was generated.

Related Experiment Videos

  • Identification of over 40 distinct polypeptide chains.
  • A table listing new and known proteins, with labels on the protein map.

    • Conclusions:

    • The updated protein map and database provide a valuable resource for researchers.
    • This work significantly enhances the catalog of identified human plasma proteins.
    • The findings contribute to a deeper understanding of plasma proteome composition.