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Towards a two-dimensional database of common human proteins.

D Burggraf1, K Andersson, C Eckerskorn

  • 1Max-Planck-Institut für Biochemie, Martinsried, Germany.

Electrophoresis
|September 1, 1992
PubMed
Summary

Researchers created a common human protein map using two-dimensional gel electrophoresis (2-DE) from five cell lines. This map serves as a foundation for a new 2-DE database, aiding future proteomic studies.

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Area of Science:

  • Proteomics
  • Human protein analysis
  • Cell biology

Background:

  • Two-dimensional gel electrophoresis (2-DE) is a key technique for protein separation.
  • Establishing a comprehensive human protein database is crucial for biological research.
  • Variations in protein expression exist across different cell types.

Purpose of the Study:

  • To construct a protein map of common human proteins.
  • To develop a foundational dataset for a new two-dimensional gel electrophoresis (2-DE) database.
  • To enable comparative proteomic analysis across different cell lines.

Main Methods:

  • Utilized two-dimensional gel electrophoresis (2-DE) on five distinct human cell lines.
  • Analyzed both total cell lysate and isolated nuclei.

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  • Employed computerized image analysis of digitized 2-DE gels to identify master protein patterns.

    • Main Results:

    • A reproducible master protein pattern was identified for each cell line.
    • A comparative analysis yielded a 2-DE protein map comprising common human proteins.
    • Initial characterization of protein spots using microsequencing, amino acid analysis, and mass spectrometry was initiated.

    Conclusions:

    • A standardized 2-DE protein map of common human proteins has been successfully constructed.
    • This map provides a valuable resource for building a comprehensive 2-DE human protein database.
    • The methodology allows for reproducible proteomic profiling and comparative analysis.