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Talin anchors and nucleates actin filaments at lipid membranes. A direct demonstration
S Kaufmann1, J Käs, W H Goldmann
1Biophysics Department, Technical University of Munich, Garching, Germany.
FEBS Letters
|December 14, 1992
Abstract:
Platelet talin nucleates actin assembly as we show here directly by using rhodamine-phalloidin labelling of actin filaments. Nucleation by talin still occurs after reconstitution into liposomal bilayers. This is also demonstrated directly after protein-lipid double labelling and light microscopic imaging. Talin, thus, is the first actin binding protein for which anchoring and nucleation of actin filament growth at lipid interfaces have been visualized.