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[Changes of bone morphogenesis proteins and transforming growth factor-beta in hind-limb bones of 21 d tail-suspended
Xin-sheng Cao1, Lian-jia Yang, Xing-yu Wu
1Department of Aerospace Medicine, The Fourth Military Medical University, Xi'an, China.
Objective:
To investigate changes of bone morphogenesis proteins (BMP), transforming growth [correction of grouth] factors-beta (TGF-beta) in tibia and the growing of femur in tail-suspended rats after 21 d simulated weightlessness.
Method:
Fourteen male SD rats were randomly divided into control group (CON) and tall-suspension group (TS). After 21 d tail-suspension, basic physical parameters of the femur were measured; changes of BMP, TGF-beta in tibia were assayed by immunohistochemical method.
Result:
During 21 d tail-suspension, all rats grew well without apparent stress reaction. After 21 d weightlessness simulation, wet weight, dried weight, ash, diameter and density of femur in TS group declined significantly (P<0.01). Immunohistochemical results of tibia showed that both BMP and TGF-beta declined in tissues from all regions of the tibia.
Conclusion:
After 21 d tall-suspension, the growth of rat's weight bearing bones were suppressed, production and secretion of BMP and TGF-beta was holdback.
