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Updated: Aug 8, 2026
A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
Published on: November 7, 2012
Temperature-sensitive mutants of the Mycobacterium plasmid pAL5000
C Guilhot1, B Gicquel, C Martín
1Unité de Génie Microbiologique, URA1300 du CNRS, Institut Pasteur, Paris, France.
Abstract:
Two plasmids were isolated as thermosensitive replicons following in vitro mutagenesis of pB4, a pAL5000 derivative mycobacteria/Escherichia coli shuttle plasmid. Plasmids pCG59 and pCG63 replicate at 30 degrees C but not at 39 degrees C. This will allow their utilisation for transposon delivery, site-specific integration, or allele exchange.
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