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Related Experiment Videos

Length polymorphisms of simple sequence repeat DNA in soybean.

M S Akkaya1, A A Bhagwat, P B Cregan

  • 1Soybean and Alfalfa Research Laboratory, United States Department of Agriculture, Beltsville Agricultural Research Center, Maryland 20705-2350.

Genetics
|December 1, 1992
PubMed
Summary
This summary is machine-generated.

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Soybean simple sequence repeat (SSR) DNA analysis revealed limited (CA)n and (GT)n repeats but abundant (AT)n repeats. These (AT)n repeats offer a promising source for developing polymorphic genetic markers in soybean.

Area of Science:

  • Plant genetics
  • Molecular biology
  • Genomics

Background:

  • Simple sequence repeats (SSRs) are valuable genetic markers in many species.
  • Soybean [Glycine max (L.) Merr.] genome characterization for SSRs is crucial for genetic studies.

Purpose of the Study:

  • To investigate the presence and extent of SSR DNA length polymorphism in soybean.
  • To evaluate the potential of different SSR types as genetic markers in soybean.

Main Methods:

  • Bioinformatic search of GenBank for SSRs.
  • Polymerase chain reaction (PCR) amplification using SSR-flanking primers.
  • Screening of a soybean DNA library.
  • Sequencing of positive clones.

Main Results:

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  • No (CA)n or (GT)n SSRs with n > 8 were found; the longest was (CA)9.
  • Five (AT)n SSRs (n=14-27) and one (ATT)n SSR (n=14-27) were detected.
  • PCR amplification revealed 6, 7, and 8 alleles at three loci, demonstrating codominant segregation.

Conclusions:

  • Soybean has a lower abundance of (CA)n SSRs compared to the human genome.
  • (CA)n SSRs are unlikely to be an abundant source of genetic markers in soybean.
  • (AT)n SSRs are abundant and suitable for developing highly polymorphic genetic markers in soybean.