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A novel method for measuring dynamic changes in cell volume.

Cristina E Davis1, Joshua J Rychak, Bouvard Hosticka

  • 1Department of Biomedical Engineering, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|November 5, 2003
PubMed
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A novel method measures cell volume changes using fluid flow in a capillary system. This technique offers continuous monitoring and avoids limitations of existing cell volume measurement methods.

Area of Science:

  • Cell Biology
  • Biophysics

Background:

  • Cell volume regulation is crucial for cellular adaptation to osmotic stress.
  • Existing methods like Coulter counters and fluorescent techniques have limitations.
  • There is a need for advanced techniques to accurately measure cell volume dynamics.

Purpose of the Study:

  • To introduce a new method for measuring cell volume changes based on fluid dynamics.
  • To overcome the limitations of current cell volume measurement techniques.
  • To validate the new method's efficacy in studying cell volume regulation.

Main Methods:

  • Cells (Cos-7) were cultured to line a glass capillary.
  • Extracellular osmolarity was altered to induce cell swelling or shrinking.
  • Differential pressure changes across the cell-lined capillary and a reference capillary were monitored.

Related Experiment Videos

  • The system measured fluid flow changes indicative of cell volume alterations.

    • Main Results:

    • The new method continuously monitored cell volume changes during rapid solution shifts.
    • It proved independent of cell morphology and maintained physiological cell-cell interactions.
    • The technique detected enhanced volume regulation in cells overexpressing phospholemman.
    • No phototoxic effects were observed, unlike fluorescent methods.

    Conclusions:

    • This fluid flow-based method provides a robust and versatile tool for cell volume measurement.
    • It offers significant advantages over existing techniques, including real-time monitoring and physiological relevance.
    • The method is suitable for studying cellular responses to osmotic challenges and the role of specific proteins like phospholemman.