Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nonlinear magic: multiphoton microscopy in the biosciences.

Warren R Zipfel1, Rebecca M Williams, Watt W Webb

  • 1School of Applied and Engineering Physics, 212 Clark Hall, Cornell University, Ithaca, New York 14853, USA.

Nature Biotechnology
|November 5, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polarization increases nuclear stiffness in macrophages despite reduction in lamin A/C levels.

npj biological physics and mechanics·2026
Same author

Obesity disrupts ovarian hemodynamics during the preovulatory and luteal phases in mice†.

Biology of reproduction·2026
Same author

Polarization Increases Nuclear Stiffness in Macrophages Despite Reduction in Lamin A/C Levels.

bioRxiv : the preprint server for biology·2025
Same author

Doppler Ultrasonography for Live Imaging and Quantification of Ovarian Vascular Function in Mice.

Journal of visualized experiments : JoVE·2025
Same author

Assessing cellular metabolic dynamics with NAD(P)H fluorescence polarization imaging.

bioRxiv : the preprint server for biology·2025
Same author

Spatially resolved charge-transfer kinetics at the quantum dot-microbe interface using fluorescence lifetime imaging microscopy.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

Frontier AI companies as biotech acquirers.

Nature biotechnology·2026
Same journal

Author Correction: Sustained nitric oxide production by engineered E. coli remodels the tumor microenvironment and potentiates immunotherapy.

Nature biotechnology·2026
Same journal

Quantum computing in transition.

Nature biotechnology·2026
Same journal

Author Correction: Improved RNA base editing with guide RNAs mimicking highly edited endogenous ADAR substrates.

Nature biotechnology·2026
Same journal

Unlocking the chemical potential of filamentous fungi using prime editing.

Nature biotechnology·2026
Same journal

A genome-scale CRISPRi perturbation atlas of human induced pluripotent stem cells.

Nature biotechnology·2026
See all related articles

Multiphoton microscopy (MPM) offers noninvasive biological imaging for deep tissue analysis in living animals. This advanced technique enables precise measurements of cellular activity and blood flow, expanding research possibilities.

Area of Science:

  • Biomedical Imaging
  • Optical Microscopy
  • Nonlinear Optics

Background:

  • Multiphoton microscopy (MPM) is a leading noninvasive fluorescence microscopy technique for biological samples.
  • Its application is rapidly growing, particularly with advancements in transgenic models and fluorescent indicators.

Purpose of the Study:

  • To highlight the capabilities and expanding applications of multiphoton microscopy in biological research.
  • To showcase MPM's utility in in vivo imaging and molecular manipulation.

Main Methods:

  • Utilizing nonlinear optics and photochemistry for advanced imaging.
  • Employing genetically encoded fluorescent indicators for cellular activity monitoring.
  • Imaging collagen via nonlinear scattering and releasing caged compounds with high spatial precision.

Related Experiment Videos

Main Results:

  • MPM enables deep tissue imaging (e.g., 500 micrometers in mouse brain) and quantification of physiological processes like calcium transients.
  • It allows for noninvasive blood flow quantification by imaging red blood cell shadows in capillaries.
  • Direct imaging of collagen fibrils and localized release of caged compounds are demonstrated possibilities.

Conclusions:

  • Multiphoton microscopy is a versatile and powerful tool for noninvasive biological research.
  • Its combination with advanced optical techniques and molecular tools significantly enhances in vivo imaging and experimental capabilities.