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Updated: Aug 30, 2026

Determination of Microbial Biomass in Soil using Digital Droplet PCR (ddPCR)
Published on: April 24, 2026
The microbial DNA cycle in soil
Laura S England1, Jack T Trevors
1Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1. lengland@uoguelph.ca
Abstract:
Upon microbial cell death and lysis in soil, the free or naked DNA is exposed to the dynamic environment of the soil. The DNA can be enzymatically degraded by nucleases (DNases), bind to soil components, genetically transform competent bacterial cells and be a nutrient for other microorganisms. In this article we discuss the dual role of DNA as genetic material and as a nutrient source in the soil environment.
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