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Updated: Aug 30, 2026
Unveiling Histone Proteoforms using 2D-TAU Gel Electrophoresis
Published on: October 18, 2024
Capillary electrophoresis analysis of histones, histone variants, and their post-translationally modified forms: a
Herbert Lindner1, Bettina Sarg, Wilfried Helliger
1Department of Medical Chemistry and Biochemistry, University of Innsbruck, Innsbruck, Austria. herbert.lindner@uibk.ac.at
Abstract:
Histones are the most intensively studied group of basic nuclear proteins and are of great importance with regard to the organization of chromatin structure and control of gene activity. The complexity of this protein family places very high demands on the analytical methods applied. The advent of high-performance capillary electrophoresis provided a promising new tool for their separation, which is an essential prerequisite for studying the biological role of this protein family. Problems inherent to histone analysis due to their unique physical and chemical properties are reviewed, and the pros and cons of distinct strategies developed for CE separation of these proteins are discussed.
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