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Updated: Aug 30, 2026

Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples
Published on: June 14, 2024
Epidemiology of leptospirosis: observations on serological data obtained by a "diagnostic laboratory for
D Cerri1, V V Ebani, F Fratini
1Department of Animal Pathology, Prophylaxis and Food Hygiene--Faculty of Veterinary Medicine, University of Pisa, viale delle Piagge, 2-56124 Pisa, Italy.
Abstract:
Serological data on leptospira infection were reported and discussed. From 1995 to 2001, the blood serum samples of 9885 domestic and wild animals and humans, living in Northern and Central Italy, were examined by the macroagglutination test (MAT) employing bratislava, ballum, canicola, grippotyphosa, icterohaemorrhagiae, pomona, hardjo and tarassovi serovars as antigens. Considering sera with > or = 1:400 antibody titers as positive, 674 (6.81%) animals scored positive. Sheep, horses, pigs and dogs gave the highest number of positive responses, particularly against the serovar bratislava and, for dogs, against icterohaemorrhagiae. The percentages of seropositivity observed in the most important animal species were: 12.13% in ovine (132 positive among 1088 tested animals), 11.40% in horses (107 positive animals among 938), 9.46% in swine (123 positive animals among 1299), 6.36% in dogs (278 positive animals among 4369), 2.39% in wild boars (11 positive animals among 459), 1.39% in deer (2 positive animals among 143), 0.48% in cattle (3 positive animals among 626). Among 250 human sera examined, 14 (5.60%) scored positive.

