In vivo multi-tissue corticosteroid microarray time series available online at Public Expression Profile Resource...

Area of Science:

• Pharmacology
• Genomics
• Bioinformatics

Background:

• Gene microarrays are crucial for analyzing gene expression changes.
• Current microarray analysis for drug response is limited to endpoint comparisons, hindering PK/PD modeling.
• PK/PD modeling requires simultaneous pharmacokinetic and time-series gene expression data.

Purpose of the Study:

• To describe unique, extended microarray time-series data for in vivo methylprednisolone response in rat liver and skeletal muscle.
• To make these data accessible online in a single gene query format.
• To facilitate PK/PD and pharmacogenomic evaluation of drug response.

Main Methods:

• Collected extended microarray time-series data from rat liver and skeletal muscle following a single methylprednisolone dose.
• Made data available online via a single gene query interface.
• Utilized existing published pharmacokinetic data and receptor models.

Main Results:

• Generated and provided online two comprehensive gene expression time series datasets.
• Demonstrated that the data can be analyzed without prior specialized microarray knowledge or software.
• Confirmed the data's suitability for PK/PD and pharmacogenomic analysis.

Conclusions:

• The presented gene expression time-series data are a valuable resource for drug response research.
• These data uniquely enable integrated PK/PD and pharmacogenomic evaluations.
• The accessibility and ease of use of the data will advance the study of drug effects on gene expression.