Sensory experiences in relation to pulpal nerve activation of human teeth in different age groups

There are no data on the correlation of intradental nerve activity and sensation from intact human teeth. We used microneurography to examine this relation and to determine whether it changes with age. Fifteen informed and healthy male volunteers were divided into three age groups: group A (18.6+/-1.82 years, mean+/-standard deviation (S.D.)), group B (38.4+/-2.70 years) and group C (64.0+/-4.06 years). Ratings of perceived pain intensity to thermal stimulation were obtained using a visual analogue scale (VAS). In addition, each subject chose one or two words from the short-form McGill Pain Questionnaire to describe perceived pain. A total of 90 single pulpal axons were studied with microneurography at the same time as the sensory experiences were recorded. Mean conduction velocities and variance estimates correlated closely with age. With advancing age, first, the percentage of teeth from which the subjects did not perceive any sensations to thermal stimulation increased, second, units responding to heat stimulus decreased, and third, latencies of sensation induced by thermal stimulation increased. In addition, a burst of afterdischarges following thermal stimulation and neural discharges evoked by thermal stimulation produced no sensation only in some of group B and C units. In contrast, no significant difference was found among three groups in VAS scores and words to describe the perceived pain to thermal stimulation. These results suggest that pulpal afferents were activated by the same mechanism(s); the hydrodynamic mechanism works immediately after thermal stimulation and is possibly followed by direct activation of some nerves, especially slow conducting fibres. In older tooth pulps, the decrease in the number of fast conducting afferents and mineral apposition of dentinal tubules impaired the nerve activation, especially by heat, as per the hydrodynamic mechanism. Spike discharges without sensation in older individuals were suggested to be due to insufficient spatial and temporal summation and may be involved with abnormal uncomfortable sensations.