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Related Experiment Videos

Multiple oscillators regulate circadian gene expression in Neurospora.

Alejandro Correa1, Zachary A Lewis, Andrew V Greene

  • 1Center for Biological Clocks Research, Department of Biology, Texas A&M University, College Station, TX 77843, USA.

Proceedings of the National Academy of Sciences of the United States of America
|November 5, 2003
PubMed
Summary

This study identified 145 clock-controlled genes (ccgs) in Neurospora crassa, revealing the circadian clock

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Area of Science:

  • Molecular Biology
  • Genetics
  • Mycology

Background:

  • Circadian rhythms are endogenous biological processes that regulate daily cycles.
  • The frequency (FRQ) protein oscillator is a well-characterized component of the Neurospora crassa circadian clock.
  • Understanding the full scope of clock-controlled genes is crucial for deciphering circadian regulation.

Purpose of the Study:

  • To comprehensively profile circadian gene expression in Neurospora crassa.
  • To identify novel clock-controlled genes (ccgs) and their functions.
  • To investigate the existence of additional circadian oscillators beyond the FRQ-based system.

Main Methods:

  • Utilized high-density microarrays for gene expression profiling.
  • Cultured Neurospora crassa in constant darkness to isolate circadian effects.

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  • Analyzed gene expression patterns in wild-type and mutant strains (frq7, frq-null).

    • Main Results:

    • Identified 145 ccgs with rhythmic mRNA accumulation, predominantly from late night to early morning.
    • ccgs are involved in diverse cellular processes including signaling, development, metabolism, and stress responses.
    • Discovered three ccgs with rhythms independent of the FRQ oscillator, suggesting a second circadian oscillator.

    Conclusions:

    • The Neurospora crassa circadian clock regulates a broad spectrum of cellular functions.
    • Evidence supports the existence of a second, FRQ-independent circadian oscillator in N. crassa.
    • This finding expands our understanding of the complexity of circadian regulatory networks.