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Immunoblot analysis of Trichomonas vaginalis antigens recognized by rabbit hyperimmune serum raised against

Khalifa E Khalifa1, Hala S I El-Wakil, Fayza S Habib

  • 1Department of Parasitology, Faculty of Medicine, Ain Shams University, Ramsis St., 11566, Cairo, Abbassia, Egypt.

Parasitology Research
|November 5, 2003
PubMed

The SDS-PAGE and immunoblot were used to identify Trichomonas vaginalis antigenic epitopes present in purified somatic and exo-antigens. The presence of common immunogenic proteins corresponding to molecular weights of 76, 60 and 23 kDa was revealed. Distinctive immunogenic bands of 92, 72, 55 and 40 kDa for the exo-antigens, and of 110, 80, 78 and 50 kDa for the somatic antigens, appeared when the antigens were probed by the homologous immune rabbit serum.

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Trichomonas vaginalis is a flagellated protozoan parasite and the causative agent of trichomoniasis, one of the most prevalent non-viral sexually transmitted infections in the United States. This extracellular parasite primarily colonizes the lower genitourinary tract in women—particularly the vagina—and in men, the urethra and prostate. Its structural and functional adaptations enable its survival, motility, and pathogenicity within the host environment.Structural Features and Host EntryT.