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Updated: Aug 30, 2026

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)
Published on: July 15, 2011
[A case of lepromatous type Hansen's disease]
Fumiaki Shirasaki1, Kazuhiko Takehara, Minoru Takata
1Kanazawa University Graduate School of Medical Science, Department of Dermatology, 15-1 Takara-machi, Kanazawa 920-8641, Japan. shirasah@med.kanazawa-u.ac.jp
Abstract:
A 68 year-old male presented with painful swelling in the extremities and disseminated small brownish nodules over his entire body. A histological examination of the skin nodules showed multiple granuloma and a thickened peripheral nerve bundle surrounded with foamy macrophages and a few lymphocytes. Fite stain revealed numerous acid fast bacilli within the cytoplasm of the foamy cells. A diagnosis of lepromatous leprosy was thus made and multi-drug therapy following the Japanese guideline with DDS, rifampicin and clofazimine was started. The clinical features improved, and the morphological index decreased after undergoing chemotherapy. Although a severe type 1 reaction developed after four months of treatment, he was treated with oral prednisolone at a dose of 40 mg/day.
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