Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cholic acid as template for multivalent peptide assembly.

Hengguang Li1, Lai-Xi Wang

  • 1Institute of Human Virology, University of Maryland Biotechnology Institute, University of Maryland, 725 W Lombard Street, Baltimore, MD 21201, USA.

Organic & Biomolecular Chemistry
|November 6, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chemoenzymatic synthesis of unusual antibody glycoforms using E. coli expressed human and bovine β-1,4-galactosyltransferase and their mutants.

Carbohydrate research·2026
Same author

The Proto Type Galectin Drgal1-L2 from Zebrafish Hinders Infection by the Infectious Hematopoietic Necrosis Virus by Binding to Its Glycosylated Receptors on the Epithelial Cell Surface.

Biomolecules·2026
Same author

Site-specific antibody labeling via endo-S2 mediated Fc glycan remodeling.

Methods in enzymology·2026
Same author

Antibody glyco-optimization and site-specific conjugation enhance the immune-stimulating activity of antibody-TLR ligand conjugates.

The Journal of biological chemistry·2026
Same author

Orthogonal Conjugation via Endoglycosidase and Microbial Transglutaminase Enables Efficient Synthesis of Dual-Payload Antibody-Drug Conjugates.

ACS chemical biology·2026
Same author

A Zebrafish Galectin-1 Isoform Is Expressed in Skin and Gills and Binds to Bacteria, Bacterial Adhesin Receptors, and Epidermal Mucus Glycans.

International journal of molecular sciences·2026

Cholic acid templates enable efficient multivalent peptide assembly for creating alpha-helix bundles. These structures show enhanced alpha-helix content and may serve as vaccine development mimics of conformational epitopes.

Area of Science:

  • Biochemistry
  • Organic Chemistry
  • Structural Biology

Background:

  • Cholic acid is an amphiphilic steroid with addressable functionalities.
  • Multivalent peptide assembly is crucial for developing novel biomaterials and therapeutics.
  • Developing rigid scaffolds for controlled peptide presentation is an ongoing challenge.

Purpose of the Study:

  • To utilize cholic acid as a rigid template for multivalent peptide assembly.
  • To synthesize cholic acid-based templates for chemoselective peptide ligation.
  • To investigate the structural properties and potential applications of the assembled peptides.

Main Methods:

  • Synthesis of cholic acid derivatives functionalized with maleimide or bromoacetyl moieties.
  • Chemoselective peptide ligation using three distinct peptides: DP178, tetanus toxoid epitope, and 2F5 epitope.

Related Experiment Videos

  • Circular dichroism (CD) spectroscopy to analyze the secondary structure of assembled peptides.

    • Main Results:

    • Maleimide-functionalized cholic acid templates demonstrated high efficiency in peptide ligation.
    • Bromoacetyl templates resulted in low ligation yields.
    • Template-assembled peptides, particularly DP178, formed stable three-strand alpha-helix bundles with increased alpha-helix content compared to single peptides.

    Conclusions:

    • Cholic acid serves as a valuable and rigid template for constructing multivalent peptide assemblies.
    • The maleimide functionalization strategy is highly effective for chemoselective peptide ligation.
    • The resulting alpha-helix bundles show potential as mimics of conformational epitopes for vaccine development.