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Allolymphocyte apoptosis induced by soluble liver specific antigen
Chang-Ku Jia1, Yan Wang, Shu-Sen Zheng
1Department of Hepatobiliary Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. jiachk@sohu.com
Hepatobiliary & Pancreatic Diseases International : HBPD INT
|November 6, 2003
Summary
Liver specific antigen induces immune responses and lymphocyte apoptosis. This suggests its potential role as a transplantation antigen for achieving liver transplant immunotolerance.
Area of Science:
- Immunology
- Transplantation Biology
- Hepatology
Background:
- Liver specific antigen (LSA) plays a role in immune responses.
- Understanding LSA alloimmunogenicity is crucial for liver transplantation.
Purpose of the Study:
- To investigate the alloimmunogenicity of liver specific antigen.
- To determine the effects of LSA on allolymphocytes.
Main Methods:
- Immunization of rats with LSA via hind footpad, portal vein, and thymus routes.
- Analysis using Western blotting, DNA gel electrophoresis, mixed lymphocyte culture (MLC), and mixed lymphocyte hepatocyte culture (MLHC).
Main Results:
- Immunization induced specific low immunoresponses and lymphocyte apoptosis.
- Increased caspase-3 expression and DNA fragmentation in splenocytes were observed.
- Mixed lymphocyte hepatocyte culture (MLHC) showed a marked decrease.
Conclusions:
- Liver specific antigen possesses alloimmunogenicity, inducing specific low immune responses and lymphocyte apoptosis.
- LSA may serve as a key transplantation antigen for inducing liver transplant immunotolerance.