Establishment of hepatocarcinoma cell line transfected by the B7 gene and its biocharacteristics
You-Zhu Li1, Quan Wang, Yan-Fang Jiang
1First Clinical Hospital, Jilin University, Changchun 130021, China. leeyz@public.cc.jl.cn
Objective:
To study the function of costimulation sign in tumor immunology and construct the new cell line B7+ Smmc7721.
Methods:
The B7 gene was transfected into the hepatocarcinoma cell Smmc7721 by liposma. Polymerase chain reaction (PCR) and reverse transcriptase-PCR (RT-PCR) were applied to test the result. MTT colorimetric assay was used to value the killing effect of lymphokine activated killer cells (LAK) activated by IL-2 on the transfected cell line and the original line.
Result:
B7+ Smmc7721 was improved to be steadily expressed B7 molecule and LAK cells could more effectively act on the B7+ Smmc7721 cells.
Conclusion:
The B7 gene can be transfected to hepatocarcinoma cells and can be expressed steadily in vitro, thus increasing the efficiency of LAK cells activated by IL-2 on them.
