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Updated: Aug 30, 2026
Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
Published on: September 15, 2016
Development of an enzyme immune assay for detecting M2 autoantibodies specific for primary biliary cirrhosis
Xiao-Hua Jiang1, Xiao-Yun Fang, Ren-Qian Zhong
1Department of Laboratory Medicine, 85th Military Hospital, Shanghai 200052, China. jhlulu@citiz.net
Objective:
To develope a new enzyme immune assay (ELISA) for detection of M2 antibody specific for primary biliary cirrhosis (PBC) by using a triple hybrid clone as antigen, which coexpresses the three immunodominant lipoyl domains of PDC-E2, BCOADC-E2 and OGDC-E2 from human sources.
Methods:
After expressing autoantigens of PBC in prokaryote by constructing recombinant expressive plasmid successfully, the fusion protein was purified by affinity chromatography. The sera of 17 PBC patients were examined. As controls, the sera of 167 non-PBC patients and the sera of 1225 normal controls aged under 28 were examined.
Results:
None of the sera from the non-PBC patients or the normal controls was positive for anti-M2 shown by the new ELISA. However, the positivity rate for anti-M2 in the PBC patients was 100% (17/17), as shown by the new ELISA.
Conclusion:
The detection system with a good sensitivity and specificity may be used as a powerful method for the diagnosis of PBC.
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