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Related Experiment Video

Updated: Jun 24, 2026

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

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The slow wound-response of gammaVPE is regulated by endogenous salicylic acid in Arabidopsis.

Kenji Yamada¹, Mikio Nishimura, Ikuko Hara-Nishimura

¹Department of Botany, Graduate School of Science, Kyoto University, 606-8502 Kyoto, Japan.

|November 6, 2003

Summary

Wounding up-regulates gammaVPE in Arabidopsis, but not through ethylene or jasmonic acid (JA). Salicylic acid (SA) enhances gammaVPE expression and reduces VSP expression after wounding.

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Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis

Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis

Published on: June 4, 2021

Area of Science:

Plant Molecular Biology
Plant Defense Mechanisms
Gene Regulation

Background:

Vacuolar processing enzyme (VPE) is crucial for vacuolar protein maturation in plants.
The Arabidopsis gammaVPE gene is upregulated in response to wounding.
Ethylene and jasmonic acid (JA) are known wound-responsive signaling molecules.

Purpose of the Study:

To elucidate the activation mechanism of gammaVPE gene expression following wounding.
To investigate the roles of ethylene, JA, and salicylic acid (SA) in gammaVPE wound response.

Main Methods:

Analysis of gammaVPE mRNA accumulation in wild-type and mutant Arabidopsis plants.
Hormone treatments (JA) and wounding experiments.
Comparison of gammaVPE and vegetative storage protein (VSP) gene expression patterns.

Main Results:

Wound-induced gammaVPE expression was independent of ethylene and JA signaling pathways.
JA treatment did not significantly affect gammaVPE expression but activated VSP.
SA-deficient mutants showed reduced wound-induced gammaVPE expression and increased VSP expression.

Conclusions:

Endogenous salicylic acid (SA) enhances wound-induced gammaVPE expression in Arabidopsis.
SA attenuates wound-induced VSP expression, suggesting a regulatory role in plant defense.
SA acts as an enhancer, not a direct wound signal, for gammaVPE and VSP regulation.