Related Experiment Videos
Isolation of a polygalacturonase-inhibiting protein (PGIP) from wheat
Gabré Kemp1, Carl W Bergmann, Ron Clay
1Department of Plant Sciences, University of the Free State, PO Box 339, Bloemfontein 9300, South Africa. gabre@ccrc.uga.edu
Abstract:
Evidence for the presence of a polygalacturonase-inhibiting protein (PGIP) from a monocotyledonous cereal is presented. A 40.3-kDa PGIP that was closely associated with the cell wall was acetone-extracted and purified from wheat (Triticum aestivum L.) leaves and stems. Wheat PGIP exhibited a highly selective inhibitory activity against endopolygalacturonase (EPG) from various fungi. Of nine EPG tested, wheat PGIP only inhibited EPG from Cochliobolus sativus, a pathogen of the tribe Poaceae. A short N-terminal amino acid sequence of wheat PGIP shows no similarity to any other characterized PGIP.