Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Correlating IgE reactivity with three-dimensional structure.

Reto Crameri1

  • 1Swiss Institute of Allergy and Asthma Research (SIAF), Obere Strasse 22, CH-7270 Davos, Switzerland. crameri@siaf.unizh.ch

The Biochemical Journal
|November 7, 2003
PubMed
Summary

This study reveals that IgE-binding epitopes on celery and cherry allergens are patient-specific. This finding challenges the effectiveness of current methods for creating hypoallergenic protein variants.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Aspergillus fumigatus</i>-specific immunoglobulin levels in BALF of CF patients.

ERJ open research·2017
Same author

Evanescence wave-based technology for the rapid and sensitive quantification of biological analytes.

The Journal of allergy and clinical immunology·2017
Same author

Interleukins (from IL-1 to IL-38), interferons, transforming growth factor β, and TNF-α: Receptors, functions, and roles in diseases.

The Journal of allergy and clinical immunology·2016
Same author

IgE Sensitization Profiles Differ between Adult Patients with Severe and Moderate Atopic Dermatitis.

PloS one·2016
Same author

Laser-assisted intradermal delivery of adjuvant-free vaccines targeting XCR1+ dendritic cells induces potent antitumoral responses.

Journal of immunology (Baltimore, Md. : 1950)·2015
Same author

Differential cytokine induction by the human skin-associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control subjects.

The Journal of allergy and clinical immunology·2014

Area of Science:

  • Biochemistry
  • Immunology
  • Allergen research

Background:

  • Investigates cross-reactive allergens Apg1.0101 (celery) and Pru av 1 (cherry), both pathogenesis-related proteins.
  • Utilizes site-directed mutagenesis and NMR spectroscopy to analyze IgE-binding capacity.

Discussion:

  • Examines the patient-specific nature of IgE-binding epitopes on these allergens.
  • Highlights the implications for understanding conformational IgE-binding epitopes.

Key Insights:

  • IgE-binding epitopes on celery and cherry allergens demonstrate significant patient specificity.
  • Conformational epitopes are crucial in determining allergenicity.
  • Current site-directed mutagenesis approaches for hypoallergenic variants may be limited.

Related Experiment Videos

Outlook:

  • Suggests a need for personalized approaches in allergy diagnosis and treatment.
  • Re-evaluates the strategy of using site-directed mutagenesis for hypoallergenic protein development.
  • Further research into patient-specific epitope mapping is warranted.