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Updated: Aug 30, 2026

Examination of Thymic Positive and Negative Selection by Flow Cytometry
Published on: October 8, 2012
Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood
Aleksandra Rauski1, Dusko Kosec, Biljana Vidić-Danković
1Immunology Research Center Branislav Janković, Institute of Immunology and Virology Torlak, Belgrade, Serbia and Montenegro.
Abstract:
The study revealed that beta-adrenoceptor blockade with propranolol (0.40 mg/100 g/day, s.c.) in adult male DA rats: (i) increased the thymocyte proliferation and apoptosis, (ii) caused disturbances in kinetics of T cell differentiation leading to distinguishable changes in relative proportion of thymocytes at distinct maturational steps and to an expansion of the most mature single positive (CD4+, CD8+) thymocyte pool, (iii) affected the relative proportion of neither CD4+ nor CD8+ peripheral blood lymphocytes (PBL), and (iv) augmented the relative number of CD8+CD25+ cells. Thus, the results suggest the role of beta-adrenoceptors in fine-tuning of T cell maturation, and, possibly, distribution and activation of distinct PBL subsets.
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