Peroxynitrite-induced changes of thiol groups in human blood platelets
Related Experiment Video
Updated: Aug 30, 2026

Alternative Methods for the Detection of Superoxide Anion Generation in Platelets
Published on: March 29, 2024
Paweł Nowak1, Beata Olas, Edward Bald
1Department of General Biochemistry, University of Łódź, Poland. pnowak@biol.uni.lodz.pl
Abstract:
In this study we investigated sulfhydryl and disulfide metabolism in blood platelets treated with peroxynitrite (ONOO-) at concentrations of 10, 100, and 1000 microM. In platelets incubated with peroxynitrite, a decrease of both protein and low molecular weight thiols (glutathione, cysteine and cysteinylglycine) was observed. The reduction of platelet free thiols caused by ONOO- was dose dependent. We found that peroxynitrite induces the oxidation of platelet free SH-groups. Platelets contain a large fraction of glutathione in reduced form (GSH). We showed that after ONOO- treatment, GSH/GSSG ratio in platelets was distinctly decreased. The presented results suggest that oxidation of thiols and depletion of GSH in platelets caused by peroxynitrite may be responsible for cytotoxic effects of this compound.
Related Concept Videos
Bioactivation and Tissue Toxicity
Formation of the Platelet Plug
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
Preparation and Reactions of Thiols

