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Related Experiment Videos

CD45RA and CD45RBhigh expression induced by thymic selection events.

V A Wallace1, W P Fung-Leung, E Timms

  • 1Ontario Cancer Institute, Department of Medical Biophysics and Immunology, University of Toronto, Canada.

The Journal of Experimental Medicine
|December 1, 1992
PubMed
Summary
This summary is machine-generated.

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Thymocyte CD45 isoform expression, specifically CD45RBhigh and CD45RA, correlates with T cell receptor selection events in the thymus. This suggests CD45 isoform modulation plays a role in thymic selection.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • CD45 protein tyrosine phosphatase is crucial for T and B cell signaling.
  • Peripheral T cells alter CD45 isoforms upon activation.
  • Mechanisms of CD45 exon switching during thymic T cell development remain unclear.

Purpose of the Study:

  • To investigate CD45 isoform expression changes during T cell development in the thymus.
  • To determine if CD45 isoform modulation is linked to thymic selection events.

Main Methods:

  • Flow cytometry analysis of thymocytes from nontransgenic and T cell receptor (TCR) transgenic mice.
  • Staining with anti-CD45RB and anti-CD45RA monoclonal antibodies.
  • Examination of CD45 isoform expression in different thymocyte subsets and selection-deficient models.

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Main Results:

  • A small population of thymocytes expressed high levels of CD45RB (CD45RBhigh).
  • CD45RBhigh and CD45RA expression correlated with positive and negative T cell selection events in TCR transgenic mice.
  • This upregulation was absent in nonselecting backgrounds and CD8-deficient models.

Conclusions:

  • Modulation of CD45 isoform expression is associated with thymic selection processes.
  • CD45 isoform switching may be a marker or mediator of thymic selection events.