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Related Experiment Videos

Cytotoxic isolates of Helicobacter pylori from peptic ulcer diseases decrease K+-dependent ATPase activity in HeLa

Awasthi Shanjana1, Ayyagari Archana

  • 1Department of Microbiology, Sanjay Gandhi P,G, Institute of Medical Sciences, Lucknow, UP, India. awasthis@uthscsa.edu

BMC Gastroenterology
|November 8, 2003
PubMed
Summary

Cytotoxic Helicobacter pylori strains are more prevalent in Indian peptic ulcer patients than gastritis patients. This cytotoxin impairs ion-transporting ATPase activity, indicating its role in disease severity.

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Area of Science:

  • Microbiology
  • Gastroenterology
  • Cell Biology

Background:

  • Helicobacter pylori is a Gram-negative bacterium linked to chronic gastritis and peptic ulcers.
  • Disease severity in H. pylori infections varies, potentially due to bacterial virulence factors.
  • Cytotoxin production by H. pylori is a key virulence factor influencing disease outcomes.

Purpose of the Study:

  • To investigate variations in H. pylori isolates from Indian dyspeptic patients based on cytotoxin production.
  • To assess the impact of H. pylori cytotoxin on K+-dependent ATPase enzyme activity in HeLa cells.
  • To correlate cytotoxin production with disease severity (gastritis vs. peptic ulcer).

Main Methods:

  • Patients were classified as having gastritis or peptic ulcer based on endoscopic and histopathological findings.

Related Experiment Videos

  • HeLa cells were exposed to broth culture filtrates (BCFs) of H. pylori isolates.
  • Cytopathic effects (morphological changes, viability) and K+-dependent ATPase activity in HeLa cells were measured.

    • Main Results:

    • Cytotoxin production was detected in a higher proportion of H. pylori isolates from peptic ulcer patients (4/4) compared to gastritis patients (3/7).
    • BCFs from cytotoxic H. pylori strains significantly reduced HeLa cell K+-dependent ATPase activity by approximately 60% compared to non-cytotoxic strains.
    • Reduced ATPase activity and cytotoxin release correlated with increased patient pathogenicity indices.

    Conclusions:

    • Cytotoxic H. pylori strains are more frequently isolated from Indian patients with severe acid peptic diseases like peptic ulcers than from gastritis patients.
    • H. pylori cytotoxin impairs ion-transporting ATPase activity, serving as a marker of bacterial cytotoxicity.
    • These findings highlight the role of cytotoxin in the pathogenesis of severe H. pylori-associated gastrointestinal diseases.