Related Experiment Video
Updated: Aug 30, 2026

Using Changes in Leaf Transmission to Investigate Chloroplast Movement in Arabidopsis thaliana
Published on: July 14, 2021
Phytochrome modulation of blue light-induced chloroplast movements in Arabidopsis
Stacy L DeBlasio1, Jack L Mullen, Darron R Luesse
1Department of Biology, Indiana University, Bloomington, Indiana 47405, USA.
Abstract:
Photometric analysis of chloroplast movements in various phytochrome (phy) mutants of Arabidopsis showed that phyA, B, and D are not required for chloroplast movements because blue light (BL)-dependent chloroplast migration still occurs in these mutants. However, mutants lacking phyA or phyB showed an enhanced response at fluence rates of BL above 10 micromol m-2 s-1. Overexpression of phyA or phyB resulted in an enhancement of the low-light response. Analysis of chloroplast movements within the range of BL intensities in which the transition between the low- and high-light responses occur (1.5-15 micromol m-2 s-1) revealed a transient increase in light transmittance through leaves, indicative of the high-light response, followed by a decrease in transmittance to a value below that measured before the BL treatment, indicative of the low-light response. A biphasic response was not observed for phyABD leaves exposed to the same fluence rate of BL, suggesting that phys play a role in modulating the transition between the low- and high-light chloroplast movement responses of Arabidopsis.
More Related Videos
07:55Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
Published on: November 20, 2017
10:10Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
Published on: May 29, 2010
Related Concept Videos
Photoreceptors and Plant Responses to Light
Biological Clocks and Seasonal Responses
Cell Signaling in Plants
The Antenna Complex
Regulation of Transpiration by Stomata
Protein Transport to the Inner Chloroplast Membrane