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Updated: Aug 30, 2026

Plaquing of Herpes Simplex Viruses
Published on: November 5, 2021
Preliminary comparison of three LightCycler PCR assays for the detection of herpes simplex virus in swab specimens
D M Whiley1, M W Syrmis, I M Mackay
1Clinical Virology Research Unit, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital and Health Service District, Herston Road, 4029 Herston, Queensland, Australia.
Abstract:
Three Herpes Simplex Virus LightCycler polymerase chain reaction assays were compared for the detection of herpes simplex virus in 48 swab specimens. The assays comprised of one in-house assay and two commercial kits: the Artus HSV LC RealArt PCR kit and the Roche LightCycler HSV 1/2 Detection kit. On the whole, the three assays had comparable sensitivities. However, differentiation of herpes simplex virus types 1 and 2 by melting curve analysis was problematic in all assays. Overall, the results highlight the limitations of typing herpes simplex virus by melting curve analysis.
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