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Updated: Aug 30, 2026
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
Expression of cyclooxygenase-1 and -2 in human breast cancer
Norio Yoshimura1, Hajime Sano, Masahiko Okamoto
1Department of Organ Transplant and Endocrinological Surgery, Kyoto Prefectural University of Medicine, 465 Hirokoji, Kawaramachi, Kamigyo-ku, 602-0841 Kyoto, Japan.
Purpose:
We investigated the expression of cyclooxygenase (Cox-1 and Cox-2) in mammary tissues from patients with breast cancer.
Methods:
We used reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry.
Results:
The cancer cells showed very weak expression of Cox-1, but strong expression of immunoreactive Cox-2. In contrast, immunoreactive Cox-2 was very weak in all of the benign mammary tumors examined, including fibroadenoma (FA) and mastopathy (MP). Immunoreactive Cox-1 was also very weak in these benign tumors. The extent and intensity of immunoreactive Cox-2 polypeptides was significantly greater in the cancer cells than in the FA cells or MP cells. RT-PCR analysis showed enhanced expression of Cox-2, but not Cox-1 in breast cancer tissue, and faint expression of Cox-2 in benign tissue.
Conclusions:
These results demonstrated that human breast cancer cells generated Cox-2, indicating that Cox-2 might play an important role in the proliferation of breast cancer cells.