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Updated: Jun 21, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
Published on: August 6, 2008
Whole-comparative genomic hybridization (W-CGH): 1. The quick overview of repetitive DNA sequences on a genome
Miguel Pita1, José Luis Fernandez, Jaime Gosálvez
1Unidad de Genética, Departamento de Biologia, Facultad de Ciencias, Universidad Autónoma de Madrid, 28049 Madrid, Spain. miguel.pita@uam.es
Abstract:
We present a technique (W-CGH) based on Comparative Genomic Hybridization (CGH), but using whole DNA probes, which permits the identification of chromosomal polymorphisms related to highly repetitive DNA sequences that exist between the two genomes compared. The procedure employs two differently colored whole DNA probes from two different individuals that are mixed and hybridized to metaphase chromosomes. The method provides a simple way to map whole genome differences for highly repetitive DNA sequences between two individuals, since it does not require chromosome-specific probes.
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