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Updated: Aug 12, 2026

Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
Published on: April 5, 2019
Characterization of gelatine and acid soluble collagen by size exclusion chromatography coupled with multi angle
Michael Meyer1, Bernd Morgenstern
1Forschungsinstitut für Leder und Kunststoffbahnen gGmbH, Meissner Ring 1-5, D-09599 Freiberg, Germany. michael.meyer@filkfreiberg.de
Abstract:
Acid soluble collagen (ASC) and a cattle hide gelatine were analyzed by size exclusion chromatography (SEC) coupled with multi angle light scattering (MALS). The SEC system was calibrated with ASC and its cyan bromide cleavage products. The accuracy of calibration was confirmed by MALS by measuring the mass-average molar masses (Mw). ASC acted as a mixture of two polymer standards of Mw = 90 and 180 kg/mol, respectively. The elution behavior of the gelatine in SEC-MALS was similar to that of ASC. Therefore, the determination of the molar mass distribution of this gelatine was possible either by SEC, using a calibration curve, or by MALS by direct measurement of Mw. According to the scaling law
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